Considerations when quantitating protein abundance by immunoblot

Considerations when quantitating protein abundance by immunoblot.

The development of the immunoblot to detect and characterize a protein with an antisera, even in a crude mixture, was a breakthrough with wide-ranging and unpredictable applications across physiology and medicine. Initially, this technique was viewed as a tool for qualitative, not quantitative, analyses of proteins because of the high number of variables between sample preparation and detection...

Quantitating translational control: mRNA abundance-dependent and independent contributions and the mRNA sequences that specify them

Translation rate per mRNA molecule correlates positively with mRNA abundance. As a result, protein levels do not scale linearly with mRNA levels, but instead scale with the abundance of mRNA raised to the power of an 'amplification exponent'. Here we show that to quantitate translational control, the translation rate must be decomposed into two components. One, TRmD, depends on the mRNA level a...

Considerations when prescribing trimethoprim-sulfamethoxazole.

famethoxazole remains a popular antibiotic because of its low cost, effectiveness and familiarity among clinicians. It is the most frequently prescribed antibiotic for urinary tract infections in Canada. Other indications include treatment of infections caused by Pneumocystis jiroveci, Toxoplasma gondii, Stenotrophomonas maltophilia and community-associated methicillin-resistant Staphylococcus ...

Ethical Considerations When Representing Organizations

Considerations When Evaluating Microprocessor Platforms

Himanshu Raj of Microsoft asked about the difference between Gatekeeper and Seawall. The primary difference is that Gatekeeper divides available bandwidth between different tenants (where each tenant has multiple VMs), whereas Seawall only allocates available bandwidth between different flows. Wenji Wu of Fermilab asked how one knows to set the limits to the minimum bandwidth required by each s...